Sampling instructions

Sampling instructions

In general - always attach the completed request form along with the labelled sample.

Please make sure to always state the referring physician´s name and phone number on the request form clearly. This is particularly important for acute testing.

Please contact the laboratory if you have any questions regarding the request forms or sampling instructions.

Telephone: +46 40 53 76 60 or analysis.se@wieslab.com

For specific sampling instructions, see below:

Autoantibody assays

Blood should be collected in plain tubes (serum tubes) without coagulants or other additives.
Both venous and capillary samples are acceptable.
3 mL serum (7 mL blood) will be enough for approximately 15 tests.
Usually one tube of serum is enough.

Serum

  • The sample should be coagulating at least 30 minutes and then centrifuged at ambient temperature
  • Separate serum into plain tubes
  • Serum should be kept cold until transport
  • Transport at room temperature, standard postal letter is acceptable (padded envelope marked UN 3373)

At times when the sample is not directly sent, the sample should be stored in a refrigerator (about 2-8 °C for up to one week) or freezer (about -20 °C for prolonged storage) awaiting transport.

Complement assays

Serum samples for complement analysis (C3, C4, C1q, and functional complement assay) should be collected in plain tubes (serum tubes) without coagulants or other additives. Serum should be aliquoted into three tubes. EDTA-plasma is needed for C3d test. The serum samples and EDTA-plasma should be frozen and shipped on dry ice.

Serum (C3, C4, C1q and functional complement assay)

  • Turn the test tube at least 5 times after sampling
  • The sample should be coagulating for at least 30 minutes and then centrifuged 10 minutes
  • Separate serum into two plain tubes and transport it on dry ice

EDTA-plasma (C3d)

  • The sample should be centrifuged for 10 minutes at ambient temperature
  • Separate plasma into a plain tube and transport it on dry ice – within 24 hours of sampling

CSF (Cerebrospinal fluid) assays

Always use polypropylene tubes for collecting, centrifuging and transportation.

Adults: Collect the first 10-12 mL CSF
Children (<16 years): Collect the first 3 mL CSF

(In case of heavy bleeding, discard the first mL CSF, and note this on the request form)

  • Samples should be centrifuged at 1300-1800g for 10 min
  • The supernatant should be transferred to a new polypropylene tube before aliquoting as described below
  • The sample must not be thawed after freezing
  • Please do not send samples on a Friday

Autoantibodies

3 mL CSF will be enough for approximately 10 tests. It is not necessary to freeze these samples before transport. The samples can be sent in padded envelopes in room temperature.

Biomarkers

The spinal fluid should be mixed well before allocation. The sample must be distributed as follows:

  • Alzheimer markers and markers of neuronal damage (Parenchymal): At least 0.5 mL CSF divided in 3 polypropylene cryotubes and frozen.
  • Neuromyelitis Optica Spectrum Disease: At least 0.5 mL CSF divided in 2 polypropylene cryotubes and frozen.

The samples must be packed with dry ice when shipped.
NOTE! Samples must not be thawed after freezing. (Never send samples on Fridays).

Narcolepsy assay

For this assay, the following samples are needed:

  • Serum: 3 mL (Trib2)
  • CSF: 0,5 mL (Orexin/Hypokretin) and
  • EDTA-plasma: 2,5 mL (HLA-DQB*06:02)

The sample should be stored in a refrigerator (about 2-8 °C for up to one week) awaiting transport. Transport at room temperature, standard postal letter is acceptable (padded envelope marked UN 3373).