dsDNA antibodies

Indication

Suspicion of systemic lupus erythematosus or incipient exacerbation of this disease, chronic aggressive hepatitis of the “lupoid hepatitis” (autoimmune hepatitis) type.

Method

IIF with the flagellate Crithidia luciliae as a substrate on a slide.

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Answer

The result is given as negative, weak, medium or strong positive after evaluation of the intensity of the immunofluorescence. A positive result is followed by a titer.

Interpretation

Analysis of antibodies against double stranded DNA (dsDNA) is performed in further investigation of positive IF-ANA, suspicion of systemic lupus erythematosus or onset of exacerbation of this disease. IgG antibodies against dsDNA is a strong marker for systemic lupus erythematosus (SLE) and is associated with SLE-nephritis but can also be seen in autoimmune hepatitis type 1.

Positive findings of these antibodies (detected by IF microscopy) have high diagnostic specificity for systemic lupus erythematosus (90%). Over time, up to 70% of patients with systemic lupus erythematosus have some time during the disease progression IgG anti-dsDNA. A rapid increase in the level of anti-dsDNA often indicates an impairment of the disease. During remission, the values may become normal.

Weak positive findings with a screening method that cannot be verified by Crithidia tests can be seen in other chronic inflammatory rheumatic diseases and are usually of low clinical value.

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Packages and other tests

View other packages ANCA for Systemic Vasculitis/anti-GBM Goodpastures syndrome (501), Glomerulonephritis (500)

Acute test